During laboratory testing for PFA-100 and platelet aggregation, he was found to platelet clumping which only developed in the sodium citrate tube, but not in EDTA. If the recollected EDTA specimen still demonstrates platelet clumping, run the citrate sample and correct the platelet result by multiplying by 1.1 for dilution correction. However did note a signal to this story is an option. Biochemical analyses. Blood Bank: Type & Screen, Transfusion, OB Panel . This is a genuine LOW platelet count that can be confirmed with both slide viewing and manual counting of platelets. He received one dose each of vancomycin and cefepime in the … Since 2002, Request A Test has been an innovator in direct-to-consumer lab testing by providing convenient, affordable, and quick online lab testing. In order to continue our current process, I am in the midst of doing a correlation between EDTA platelet counts vs. citrated counts. If you obtain a platelet count from a blood sample collected in a sodium citrate tube, the result is multiplied by 1.1 to correct for the volumetric difference in anticoagulant compared with EDTA. The choice of the concentration depends upon the policies of the laboratories. EDTA (lavender-top) tube. The degree of inaccuracy was greater for platelet counts under 20 x10(9) plt/L. Outcomes The patient's platelet counts returned to normal with the use of sodium citrate-anticoagulated blood samples. Collection Instructions. Results for "platelet count" showing 1-10 of 283. Collect blood in 3 sodium citrate (light blue-top) tubes; tubes must be filled to capacity to insure correct blood to anticoagulant ratio. Maintain specimen at room temperature. Introduction. Depends on what we’re testing for. Collection Tube. the appropriate concentration of citrate anticoagulant in the plasma to be tested. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. This laboratory test is available in 1 online lab test stores. Refrigeration can precipitate fibrin and trap WBC and platelets. If specimen will be analyzed after 24 hours: Centrifuge the citrated blue-top tubes at designated speed and time to obtain platelet-poor plasma (10,000/µL).Using a plastic pipette, remove 1 mL of plasma and transfer to a plastic aliquot tube and … 2.7 mL blue-top (sodium citrate) tube. Collection Timing : Mon-Sun: 7:30am-8pm Address : Shop No- 10, First Floor, I- Block, Sector-12, Noida Molecular Genomics: See Test Catalog. 1 mL platelet-poor plasma collected in each of three separate 3.2% sodium citrate (light-blue top) tubes. To identify and help diagnose platelet dysfunction in those with a history of excessive bleeding. Platelet Count, EDTA test cost is between $23.00 and $29.00. Causes may include: A type of anemia in which red blood cells in the blood are destroyed earlier than normal (hemolytic anemia) Iron deficiency. Inadequate filling of the collection tube will decrease this ratio, and may lead to inaccurate results. • Buffered sodium citrate 0.105 M (≈3.2%) glass 0.109 M (3.2%) plastic • Citrate, theophylline, adenosine, dipyridamole (CTAD) 3-4 3-4 For coagulation determinations. Reference ranges are provided as general guidance only. Blood Bank: Type & Screen, Transfusion, OB Panel . Presenilin 1 Gene. Sodium Citrate (Lt Blue) Coagulation: PT/INR, APTT, Factor Assays, Fibrinogen, D-Dimer ... Platelet Count, Reticulocyte Count, ESR(Sed Rate) Hemoglobin A1C, BNP . Molecular Genomics: See Test Catalog. If either platelet satellitism or platelet clumping is observed on the peripheral smear, the sample could be recollected using sodium citrate as the anticoagulant. Questdiagnostics.com DA: 24 PA: 13 MOZ Rank: 47. 1. Sol A is a solution of 22 g/L trisodium citrate, 8 g/L citric acid, and 24.5 g/L dextrose. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. ANDA BA125608 / Sodium Citrate 4% w/v Anticoagulant Solution USP 18.104.22.168.2 Draft Package Insert Labeling Text Terumo BCT, Inc. 10811 West Collins Ave. Lakewood, Colorado 80215-4440 USA This is because of most common finding has been shown to reduce the sticking together. The repeat labs showed platelet count of 9,000/mm 3, and later 4,000/mm 3 in citrate tube. Pediatric Reference Intervals, Eighth Edition, is a must-have for clinical chemists, hematologists, pathologists, endocrinologists and pediatricians. PLATELET COUNT,SPECIAL test cost minimal is in Personalabs (Platelet Count, Citrated Blood Test) with price $51.00. Plasma Platelet Poor Plasma: o Specimens are drawn into tubes that contain anticoagulant. The first sodium citrate tube was discarded to minimize the chance that endothelial and platelet activation from the initial venipuncture would result in spurious biomarker readings. When you result the platelet count from the sodium citrate … This testing will assist in determining the cause of platelet clumping. 5 mL frozen platelet-poor citrated plasma collected in a 3.2% sodium citrate (light-blue-top) tube. Prealbumin. This removes the defense mechanism against the damage. Centrifuge whole blood at 1500 g for 15 minutes. CTAD tubes contain besides the buffered citrate Platelet Function Test Lithium HIV 1 DNA, Qual, PCR T/F Protein C Activity Lysozyme (Muramidase) OR HIV Viral Load T/F = Transfer plasma to plastic vial - T/F Protein C Ag (Total) Mellaril (Thioridazine) Mint Green (PST gel separator) OR Dark Green (lithium heparin), Gold, Tiger Top are also acceptable T/F HSV 1 and 2 PCR, Qual … Inversions ensure mixing of clot activator with blood. There are tests that determine your blood quality, such as hemoglobin, RBC count, WBC count, MCV, MCH, MCHC, Platelet count, PBS and so … These bleeding or clotting disorders or bone marrow diseases require the determination of the number of platelets present and/or their ability to function correctly. Citrated plasma is also used to measure coagulation-relevant factors. Use: Measures the degree of platelet function in patients who are taking the thienopyridine class of drugs including clopidogrel and prasugrel. Citrate) or 1.8 mL Clear/Blue top (3.2% Na. The proportion of blood to the sodium citrate anticoagulant volume is 9:1. It means your body is making too many platelets. Blue-top (sodium citrate) tube Collection Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. $23.00. Off-campus laboratory: Two blood smears are to accompany sample. A high platelet count is 400,000 (400 × 10 9 /L) or above. Sol B is made up of 13.2 g/L trisodium citrate, 4.8 g/L citric acid, and 14.7 g/L dextrose. Sodium Citrate Platelet Count. Centrifuge light blue-top tube for 15 minutes at approximately 1500 g … Platelet Count Verification by Citrate Anticoagulant. He was transfused with 1 unit of apheresed platelet with resultant rise in platelet count to 21,000/mm 3. Step 1: Add to cart - … Platelet … The amount of citrate concentration depends on a laboratory’s policies. For patients who repeatedly have platelet clumps which preclude determination of an accurate platelet count, it is now possible to order a platelet count from a citrate tube. In many cases the EDTA in a lavender top tube causes the platelet clumping which does not occur in a blue top (citrate tube). The Epic order code is LAB7882. Each 100 ml solution of CPD-A1 contains: 2.63 g sodium citrate, 0.30 g citric acid, 0.22 g sodium biphosphate, 3.19 g dextrose and 27.5 mg adenine. April 2020—Siemens Healthineers announced it was selected by Quest Diagnostics to be Quest’s preferred supplier for immunoassay testing. High Hematocrit Samples. 1 Evacuated collection tubes must be filled to completion to ensure a proper blood-to-anticoagulant ratio. Traumatic draw can introduce thromboplastin and trap WBC and platelets. Clotted, frozen, hemolyzed, overfilled, underfilled, or centrifuged samples. Results for "platelet count" showing 1-10 of 283; Platelet Count, EDTA - Platelets must be present in adequate numbers and have proper function to aid in hemostasis; Platelet Antibody, Direct, Flow Cytometry - Thrombocytopenia that is refractory to platelet … SPECIAL INSTRUCTIONS: Collect 1-Blue top tube and 1-Lavender top tube for testing. Pseudothrombocytopenia (PTCP) is an in vitro phenomenon of platelet aggregation that results in spurious reporting of a low platelet count by automatic cell counters, which are typically EDTA dependent. George Fritsma. Thrombin Clotting Time 883 TT 1 mL frozen platelet-poor plasma (3.2% sodium citrate [light blue-top tube]); 0.5 mL minimum von Willebrand Disease Test Name Test Codes Aliases Panel Components Specimen Requirements Factor VIII Activity, Clotting 347 FVIII activity, functional FVIII, hemophilia A test 1 mL frozen platelet-poor plasma (3.2% sodium citrate Pregnenolone. The Epic order code is LAB7882. Container: Sodium Citrate (light blue) Minimum Volume: 0.5 mL. Traumatic draw can introduce thromboplastin and trap WBC and platelets. Minimum Volume. Platelet-poor plasma (PPP) was prepared by centrifugation at 1500g for 15 minutes and stored … Collection Instructions. Patients with elevated … She had a complete blood count performed using a standard EDTA tube and she had a low platelet and elevated lymphocyte count. Minimum Volume. 0.5 mL each specimen. (ONLY for patients where the platelets clump in EDTA anticoagulant) Do NOT centrifuge. PPT (Pearl) Molecular Diagnostics: HCV/HBV/HIV PCR Quant (viral load) … Find A Doctor. NO CLOTTING TIME REQUIRED PT/INR PTT FIBRINOGEN DDIMER SPECIAL COAG & FACTOR ASSAYS CALL COLLECTION PFA- 2 GLASS 4.5ML TUBES DO NOT SEND VIA TUBE … CPT coding is … o Occasionally a Red top tube is required of the whole blood specimen. Check it with a CITRATE sample (to get a count … Collection Instructions. In EDTA the platelet count is approximately 130–140K but drops to 50–80K in citrate … Blood was collected from either the femoral or saphenous vein into two 2.9-mL sodium citrate (3.2%) (Sarstedt, Nümbrecht, Germany) using the 2-tube technique. Room temperature. Home. EDTA-treated whole blood was used for platelet count assessment via automated clinical analyzer (Quest Diagnostics Inc. Willimantic, CT). 10, 000) and accurate coagulation test results. A platelet count will be performed on a lav top EDTA tube and on a blue top sodium citrate tube. Room temperature: 48 hours. Quest Diagnostics: Test Directory. PLATELET COUNT. Specimen required: 2 mL EDTA lavender-top tube minimum 1.6 mL of whole blood or EDTA microcollection tube with 250-500 uL of whole blood. For EDTA platelet clumping ONLY, draw an additional 2.7 mL blue-top (sodium citrate) tube. Sodium Citrate (Lt Blue) Coagulation: PT/INR, APTT, Factor Assays, Fibrinogen, D-Dimer ... Platelet Count, Reticulocyte Count, ESR(Sed Rate) Hemoglobin A1C, BNP . No. ... (LabCorp) and Hartford HealthCare in Connecticut (Quest Diagnostics) this year, for example, and MemorialCare in California (Quest) in 2015. Sodium Citrate Gel barrier 2 EDTA Lithium Heparin Gel barrier Gold Plasma Separator Tube (PST) • All monthly serum chemistry test(s) including Sodium, Potassium, Chloride, Bicarbonate, BUN/Creatinine, Iron and Lipids • T3-T4-TSH3 • Ferritin • Vitamin B12 ... Diagnostics Specimen Collection Guide Greiner Rev.6 … They must be labeled with two (2) patient identifiers and made within eight (8) hours of collection. Sodium Citrate (Light Blue) For coagulation and platelet function tests. This updated … This phenomenon has occurred on at least 3 visits. Use only siliconized or plastic collection tube to collect blood (follow NCCLS guidelines H3-A3 and H21-A2). CMV Antibody None 2d B12 SST 2d Platelet Count None 2d Cortisol None 2d Beta-HCG SST 2d Reticulocyte count None 2d ... LIGHT BLUE – SODIUM CITRATE PINK - EDTA Questions? Quantitative platelet disorders have varied etiology. CONTAINER TYPE: Blue top (sodium citrate) tube and Lavender top (K2 EDTA) tube - 3 mL or Lavender top (K2 EDTA) tube - 6 mL. COLLECTION: SPECIMEN REQUIREMENTS: Blood. 1. Platelet Count, EDTA test cost max is in True Health Labs (Platelet Count, Citrated Blood) with price $49.00. Centrifuge for at least 15 minutes at 2200-2500 RPM. 20 microlitreof blood is taken in a pipette & 1.98 ml of diluent is added.platelets are counted in NeubeurHemacytometer. Eosinophil Count, Nasal (EOSN/Quest) Eosinophil Count, Sputum (EOSS/Quest) Eosinophil ... Platelet Count Platelet Function Test PNH, PI-Linked Antigen, Blood Protein C Activity Protein C Antigen ... Molecular Diagnostics. In our laboratory, if vortexing does not resolve platelet clumping, we request the specimen be recollected in both EDTA and citrate tubes. 2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the … Please submit a separate, frozen vial for each special coagulation assay ordered. Prenatal Group B Strep (GBS) Screening. For platelet-rich plasma (PRP) whole blood was centrifuged at 152 g for 10 min at 23°C and for platelet-poor plasma (PPP) at 1500 g for 15 min at … • 0.150M sodium citrate (3.2%) 3-4 inversions Light Blue • Clotctiv a ator and gel for serum separation 5 inversions Serum Separator Tube (SST®) for serum determinations in chemistry and serology. Automated Multiparameter Hematology Analyzer. False positive results may be due to immune complexes. 2. Like EDTA, citrate acts by removing calcium from the blood. In response to dramatic changes in healthcare delivery systems that have come as a result of the aggressive cost-cutting measure of managed care, we have now expanded our circulation to include all categories of professionals responsible for the safety of employees who perform specimen collection and handling. LabNotes. The count will probably be flagged by the analyzer for platelet clumps or giant platelets. If specimen will be analyzed within 24 hours: Whole blood can be submitted. Platelet Antibody, Direct, Flow Cytometry - Thrombocytopenia that is refractory to platelet transfusions may be due to direct platelet … PLATELET COUNT,SPECIAL test cost is between $51.00 and $51.00. Since the additive still results in a whole blood sample — plasma and red blood cells, the coagulation tubes also contain buffered tri-sodium citrate solution. Do not refrigerate. Sodium citrate (0.102 M, 0.129 M citrate, buffered and nonbuffered) at a ratio of nine parts blood to one part anticoagulant is the anticoagulant typically chosen for platelet aggregation testing. The final citrate concentration in the blood should be adjusted in patients who have hematocrit values above 55%. Specimen Stability. PRA. Heparin-Induced Platelet Antibody - Heparin can induce thrombocytopenia, a decreased platelet count, due to antibody stimulation. 2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure … The platelet count is Contains separator gel and should not be used for toxicology or drug testing. Inversions ensure mixing of clot activator with … Spectrum Health Lakeland. Collection Instructions: Patient prep: Overnight fasting is preferred. Refrigeration can precipitate fibrin and trap WBC and platelets. This manual platelet count is cross checked with the platelet count obtained by Giemsa stained smears. Citrate concentrations of either 0.109 mol/l (3.2 %) or 0.129 mol/l (3.8 %) are available. PR3-ANCA. If we wanna see evidence of bacterial infection, pop a slide of the blood in question under a microscope and look for neutrophils. Test Code LAB90128 Platelet Count (Citrate), Blood Reporting Title. Quest Diagnostics : Search Results. Assay performance is affected by hematocrit values less than 33% or greater than 52%, and platelet counts less than 119 x 10 9 /L or greater than 502 x 10 9 /L. Platelets can then be counted using the automated method. ... lithium or sodium heparinized, sodium citrate), is available on the company’s Advia Centaur, Centaur XP, and Centaur CP immunoassay systems. Contains separator gel and should not be used for toxicology or drug testing. For patients who repeatedly have platelet clumps which preclude determination of an accurate platelet count, it is now possible to order a platelet count from a citrate tube. Home Collection Available. Locations. False negative results may be due to low titer, low avidity antibodies. The platelet counts were determined with a Coulter Ac Tdiff cell counter (Beckman Coulter, Madrid, Spain). Buffered sodium citrate 0.105 M (≈3.2%) glass 0.109 M (3.2%) plastic 3-4 tube inversions ensure proper mixing of anticoagulant with blood. The platelet count in EDTA samples was either stable (less than a 5% increase in 11 samples) or increased at most by 12 %. Specimen Collection: Blood Container(s): 4.5 mL Blue top (3.2% Na. Room temperature. CTAD for selected platelet function assays and routine coagulation determination. This laboratory test is available in 3 online lab test stores. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Blood should not be collected from heparinized lines. Baseline citrate counts were lower than concurrent EDTA counts in 13 patients, with citrate count ranging from 84% to 102% of EDTA count. Criteria for excluding potential blood donors include low platelet count (< 150 × 10 3 /μL) and use of any drug known to affect platelet functions in the 72 hours prior to the … This trusted source enhances interpretation of patient results, allows comparison of test results using different methods, and helps optimize patient care. So, before reporting that platelet count as LOW: Check the sample for micro clots. 1 Evacuated collection tubes must be filled to completion to ensure a proper blood-to-anticoagulant ratio. full blood count (Coulter counter LH750 with software integrated normal ranges; Beckman Coulter, Lane Cove, New South Wales [NSW], Australia), and additional sam-ples were collected into citrate for platelet testing and CTAD tubes (sodium citrate, theophylline, adenosine, and dipyridamole) for electron microscopy. Microtainer 0.5 mL. In a case where citrate didn’t work, George substituted heparinized tubes. • 0.150M sodium citrate (3.2%) 3-4 inversions Light Blue • Clotctiv a ator and gel for serum separation 5 inversions Serum Separator Tube (SST®) for serum determinations in chemistry and serology. 1 Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio. I have found there to be a 20–25% difference between the two values, If multiple draw, collect EDTA (lavender-top) tube last. 2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the … To determine the blood quality, you must not centrifuge the blood sample. Note: Please examine specimen collection and transportation supplies to be sure they do not include expired containers. Keep in minerals such as aspirin therapy and obstruction kidney infection of skin irritation skin cancer and larger. Platelets, also called thrombocytes, are tiny fragments of cells that are essential for normal blood clotting. 198 Platelet Count Stability in Sodium Citrate . If present consider a recollection advising phlebotomy to collect a sodium citrate specimen in addition to the normal EDTA specimen in case of EDTA sensitivity in the patient. Medical … Collect in an appropriate collection tube. If drawn through an indwelling catheter, flush with 5 mL of saline and discard the first 5 mL of blood collected before collecting the specimen for coagulation testing. The manual platelet count is done by using 1% ammonium oxalate as diluent( for lysing RBC) with 1:100 Dilution. From Susan Lemire, Elliot Hospital, New Hampshire Hi George, I am validating the new Sysmex XN analyzer and they do not support a citrated platelet count for clumped platelets. Blood was drawn into Vacutainer blue stopper tubes (Becton Dickinson, Franklin Lakes, NJ) containing 3.8% sodium citrate (9:1 vol/vol). Becton-Dickinson only recommends substituting sodium citrate tubes for EDTA tubes. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. The following analyses were done on both samples: platelet count, hematocrit percent, hemoglobin concentration, prothrombin time (PT), activated partial thromboplastin time … She conducted an information-laden data review of the current GTI PF4 IgG/M/A kit with their new PF4 IgG kit, serotonin release assays (SRA) provided by Dr. Jeffery Dlott at Quest Diagnostics, and the 4T clinical and platelet count rating system. 3.2% Sodium citrate (lt.blue-top) preferred. Baseline platelet counts ranged from 58 to 607 x 10 9 /L. But in diagnostics, an uncredited or off-label use of an assay might be its main use—possibly even its most clinically important use. A higher-than-normal number of platelets is called thrombocytosis. REJECTION CRITERIA: Specimen clotted, tube not filled. Centrifugation: When plasma is required, or when not using a serum gel separator tube, follow these instructions: Draw 12 mL of whole blood for each 5 mL of serum or plasma needed. They are formed from very large cells called megakaryocytes in the bone marrow and are released into the blood to circulate. It is … Furthermore, the phenomenon of EDTA-PTCP disappeared when the patient was cured. The best anticoagulant to count platelets in normal human blood is EDTA sodium (Na) or Potassium (K). PLATELET COUNT FOR PATIENTS WITH PLATELET CLUMPING. Quest Diagnostics- Noida Sector 12. Methodology. 1 Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio. Transport Temperature. Platelet-poor plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Citrated plasma samples should be collected by double centrifugation. In the morning of last day of aspirin treatment, thus typically 2–4 h after aspirin ingestion, blood was drawn in 3.2% sodium citrate tubes (Greiner) for platelet function testing, in PAXgene Blood RNA tubes (BD) for genome-wide RNA profiling, and in BD Vacutainer K2 tubes for complete blood counts with … 1 mL whole blood EDTA (lavender-top) tube. Contact University of Colorado Hospital Clinical Laboratory at 720.848.4401 Preferred Specimen: 1 mL frozen platelet-poor plasma collected in 3.2% sodium citrate (light blue-top) tube. Unlike EDTA, it’s reversible—so calcium can be added back to study coagulation under controlled conditions. Citrate) Preferred Volume to Collect: 4.5 mL (Tube must be full) Minimum Volume to Collect: 1.8 mL (Tube must be full) Capillary collect ok: No Microtainer acceptable: 1.8 mL clear/blue top must be full Collection Instructions: A small whole blood clot or very small fibrin clots in the EDTA anticoagulated specimen will usually be associated with clumping of platelets on the slide, and with a false low platelet count. The CPT codes provided are based on AMA guidance and are for informational purposes only. Blood was collected from either the femoral or saphenous vein into two 2.9-mL sodium citrate (3.2%) (Sarstedt, Nümbrecht, Germany) using the 2-tube technique. Using a 21G needle, blood was drawn by venipuncture into 30 ml syringes containing either acid citrate dextrose (ACD-A) in a 1:5 volume ratio for experiments carried out in washed platelets, or sodium citrate 3.2% in 1:9 volume ratio for experiments in platelet-rich plasma (PRP). Platelet Count, EDTA - Platelets must be present in adequate numbers and have proper function to aid in hemostasis.... More. CLSI recommends that sodium citrate tubes be spun at 1500 RCF for 15 minutes to achieve platelet poor plasma (platelet count . Oct 6, 2016 10:20am. Both increases and decreases in the platelet count can point to abnormal conditions of excess bleeding or clotting. Plasma and platelet poor plasma were then aliquoted, snap-frozen, and stored at −80 °C until later analysis. PR3. The platelet count in EDTA samples was either stable (less than a 5% increase in 11 samples) or increased at most by 12 %; Platelet count in citrate samples always decreased over time, with % difference between baseline and 4 … Academic.oup.com DA: 16 PA: 37 MOZ Rank: 76. SODIUM CITRATE 4% W/V ANTICOAGULANT SOLUTION USP is designed to be metered by an apheresis device in. apheresis procedures, to prevent platelet activation and coagulation as blood moves throughout the extracorporeal unit (tubing set) in an apheresis procedure. EDTA lavender-top tube or microcollection tube. Platelets are often clumped on smears obtained from capillary blood, contributing to imprecision. Blood from patients in DMP 754-010 studies was collected into 1:10 volume of 3.2% sodium citrate. An interpretation will be provided based upon the results of the platelet counts. When using a winged blood collection set for venipuncture and a coagulation (citrate) tube is the first specimen to be drawn, a discard tube should be drawn first. Venous blood (9 ml) for platelet aggregation studies was collected from the antecubital vein by gentle aspiration using a 21-gauge butterfly cannula and a 10-ml sterile syringe charged with 0.105 M sodium citrate anticoagulant 1 ml. For home collection - give a missed call 8080444255. The first sodium citrate tube was discarded to minimize the chance that endothelial and platelet activation from the initial venipuncture would result in spurious … Room temperature: 24 hours. Performing Laboratory. 2.5 mL. As an anticoagulant, citrate binds the calcium in blood that is needed for clotting. Many times a platelet count … Make a blood film and check if they are really low. Transport Container. The platelet count obtained from the sodium citrate tube must be multiplied by 1.1 to account for the different blood-to-anticoagulant ratio in the citrate tube. HEXAGONAL PHASE CONFIRMATION - Hemolyzed specimens are not acceptable. Platelet Count, EDTA test cost minimal is in Lab Testing API (Platelet Count, EDTA Blood Test) with price $23.50. Platelet Counts from Citrate Tubes. Tube inversions ensure mixing of anticoagulant (citrate) to prevent clotting. In many cases the EDTA in a lavender top tube causes the platelet clumping which does not occur in a blue top (citrate tube). Find a Doctor Find a Location Call 800.968.0115.